Eyre & Spottiswoode, Ltd was the London-based printing firm that was the King's Printer, and subsequently, after April 1929, a publisher of the same name. It became part of Associated Book Publishers and merged with Methuen Publishing in the 1970s.

William Strahan established his printing house in London in 1739 and by 1769 had a share in both the King's Printing House and the Law Printing House. George Edward Eyre and Andrew Spottiswoode were printers to the Queen's most excellent majesty for Her Majesty's Stationery Office in 1845. Their sons subsequently ran the business. The firm was re-appointed King's Printer after the accession of King Edward VII in May 1901.^[1]

In the 19th century the firm had a printing works at Shacklewell.

The 2nd City of London Rifle Volunteer Corps was founded in 1860 as one of many such regiments raised in response to an invasion scare. Recruited in the Fleet Street area, largely from Eyre & Spottiswoode's printing works, it was known as "the Printers' Battalion". Among the first officers to be commissioned into the unit were George A. Spottiswoode and William Spottiswoode.^[2][3][4] When the unit became the 6th Battalion London Regiment (City of London Rifles) in the new Territorial Force in 1908, G Company was still mainly recruited from the company's employees.^[5]

In 1920 the firm experienced the dubious distinction of being the first to publish, in printed book form, the notorious antisemitic text The Protocols of the Elders of Zion, with the additional title The Jewish Peril.

However, as Norman Cohn points out, a distinction is to be made between the printer and the publisher of the same name. The book, or rather pamphlet, shows it was printed by "EYRE & SPOTTISWOODE, LTD."^[6]