Disney's House of Mouse is an American animated television series, produced by Walt Disney Television Animation (now Disney Television Animation), that originally aired for three seasons from 13 January 2001, to its finale on 24 October 2003. The show focuses on Mickey Mouse and his friends running a cartoon theater dinner club in the fictional ToonTown, catering to many characters from Disney cartoons and animated movies while showcasing a variety of their cartoon shorts. The animated series is a spin off of the popular short-lived Disney series Mickey Mouse Works, and featured many Mouse Works shorts as well as selection of brand new shorts; classic Mickey Mouse, Goofy and Donald Duck shorts from the 1930s–50s were also occasionally seen, particularly towards the end of the series' run when the finite amount of Mouse Works shorts had almost been exhausted.

During its time, the animated series held two nominations for awards, while select cast members won two awards for their performances as characters in House of Mouse. The series featured two special films – Mickey's Magical Christmas: Snowed in at the House of Mouse and Mickey's House of Villains – along with an all-night marathon of the House of Mouse, aired on Toon Disney in September 2002 under the title "Night of 1,000 Toons".

The basic premise of the show focuses on Mickey Mouse and his friends operating a dinner theater club in downtown ToonTown. Considered a popular venue by the residents, the club is frequented by a host of character from Disney animated properties – every character from cartoons and films produced by Walt Disney Animation Studios are featured in the episodes, with the exception of those made after 2001–2003 (such as Lilo and Stitch). Such characters mostly appear as paying guests of the club, with a few voiced in episodes depending on the scripts provided to voice actors, although a number sometimes operate as performers for the club. The animated series is more notable for including many relatively obscure and otherwise rarely used Disney characters, often with speaking parts for the very first time - for example, Li'l Bad Wolf and April, May and June, who had appeared very often in Disney comic books but never before in an animated cartoon, finally made their animated debuts on House of Mouse. The show also featured some cameos by characters created for other television cartoons and theme park attractions, but these appearances were few and far between.

Each episode focuses on a story involving Mickey and his associates facing an issue during an evening's operation of the club, and their efforts to overcome it – the most common plot for episodes involves the group dealing with a serious problem caused by Pete in his attempts to shut down the club and use it for his own gains. These stories, often involving farcical mishaps, tended to act as a wraparound for the cartoon shorts played in between scenes, the theme of the story contributing towards the story-lines of the cartoon shorts shown in the episode.^[1] Cartoon shorts played in episodes focused on elements from classic theatrical cartoons of the 1930s, 1940s and 1950s, though most were reruns from Mickey Mouse Works, and featured a simple story. Some stories were set to a specific theme for a specific character, but with differing scenarios – for example, one set focused on Mickey seeking to rescue Minnie from Pete, against a different obstacle in each short, while another set focused on Pluto's efforts to get Mickey his paper while facing a different problem.

The show was produced by Walt Disney Television, and originally aired from 2001 to 2003, running for 52 episodes. The show is one of many Disney cartoon series made in the widescreen HD format. Like with Mickey Mouse Works, the animation was outsourced to Toon City in the Philippines. Unlike that series, it was also outsourced to Walt Disney Animation Australia and Walt Disney Animation Japan.

House of Mouse aired on One Saturday Morning on ABC. It reran from September 3, 2002 to February 4, 2006 on Disney Channel. The show ceased broadcast on U.S. television on February 6, 2009, after being aired for the last time on Toon Disney before becoming Disney XD.

The theme song is performed by Brian Setzer.