Tsubomi (蕾) is Japanese for a flower bud. It may refer to:
- Tsubomi, a character in the visual novel Shuffle!
- Tsubomi Okuwaka, a character in the light novel series Strawberry Panic!.
- Tsubomi Hanasaki, a character in the anime HeartCatch PreCure!
- Tsubomi Kido, a character in the light novel series Kagerou Project
- Tsubomi (magazine), a Japanese manga magazine