Chambers is known for such television soap opera roles as Rita Merrick in The Restless Years, Dr Robyn Porter in The Young Doctors, and Rosemary Daniels in Neighbours. Chambers has played Rosemary on a recurring basis since 1986. Chambers made a brief return to the show in 2005, after line producer Linda Walker emailed her asking if she would return for the 20th anniversary celebrations. She shot her scenes in April on a visit to Melbourne. In May 2010, it was announced that Chambers would again be reprising the role of Rosemary.
Chambers has a keen interest in poetry and literature which began in childhood, with her father encouraging her deep love of the English word. Chambers is an author of historical novels. She is also the International Patron of the Ipswich Poetry Feast.